INVESTIGADORES
ROSSI juan pablo Francisco
artículos
Título:
Reversal of the Ca2+-pump in human red cells
Autor/es:
1. ROSSI J.P.F.C., GARRAHAN, P.J., Y REGA, A.F
Revista:
JOURNAL OF MEMBRANE BIOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 1978 vol. 44 p. 37 - 46
ISSN:
0022-2631
Resumen:
Reversal of the calcium pump in human red cells.
Rossi JP, Garrahan PJ, Rega AF.
Abstract
Human
red cells containing low ATP and high Pi concentrations were suspended
in media with and without 2 mM Ca2+, and the incorporation of (32P)Pi
into ATP was measured. There was some incorporation whatever the medium,
but in every experiment there was an extra incorporation when the cells
were in the Ca2+-containing medium. This extra incorporation was
abolished by the ionophore A23187, which collapses the Ca2+
concentration gradient across the membranes, or by LaCl3, which blocks
the Ca2+ pump. Starved and phosphate-loaded cells also show an uptake of
Ca2+ which is not apparent in fresh cells. Results are consistent with
the idea that Ca2+-dependent incorporation of Pi into ATP is catalyzed
by the Ca2+ pump using energy derived from the Ca2+ concentration
gradient.