INVESTIGADORES
URDAMPILLETA juan Domingo
artículos
Título:
Karyotype evolution and phylogenetic analyses in the genus Cardiospermum L. (Paullinieae, Sapindaceae)
Autor/es:
URDAMPILLETA, J. D.; COULLERI, J. P.; FERRUCCI, M. S.; FORNI MARTINS, E. R.
Revista:
PLANT BIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2013 vol. 15 p. 868 - 881
ISSN:
1435-8603
Resumen:
Cardiospermum L. belongs to the Paullinieae tribe (Sapindaceae) and comprises 16 species. Of these, 12 species are present in South America and all occur in Brazil. Car- diospermum shows the most variable chromosome number of the tribe. Phylogenetic relationships within the genus Cardiospermum, especially with other species of the tribe, are poorly understood. This research focuses on characterisation of the karyo- typic features of Cardiospermum using conventional cytogenetic methods, CMA/DAPI chromosome banding and fluorescence in situ hybridisation (FISH). To elucidate the phylogeny of the genus, the nuclear markers ITS1 and ITS2 were sequenced and analy- sed using maximum parsimony and Bayesian inference. Cardiospermum shows impor- tant diversity in basic numbers, with x = 7, 9, 10, 11 and 12. All species studied have metacentric and submetacentric chromosomes, some species have subtelocentric chro- mosomes, while telocentric chromosomes are absent. The interphase nuclei differenti- ate the Cardiospermum species into two groups. The CMA3/DAPI chromosome banding revealed the presence of an AT-rich terminal region in C. corindum, C. gran- diflorum and C. urvilloides, whereas GC-rich regions were found in C. grandiflorum, C. halicacabum var. halicacabum, C. halicacabum var. microcarpum, C. heringeri and C. integerrimum. FISH revealed syntenic and non-syntenic distribution of the 18-5.8- 26S and 5S rDNA. The syntenic distribution always occurred in the short arms of the same chromosome in all of the species. The phylogenetic relationships reveal, in part, the taxonomic arrangement of the genus Cardiospermum.