Effect of glucose and reactive oxygen species on boar sperm induced-acrosome exocytosis

MELINA FAGGI; VANZETTI AGUSTIN; JUAN MANUEL TEIJEIRO

RESEARCH IN VETERINARY SCIENCE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2023

0034-5288

Ejaculated boar spermatozoacan be liquid preserved for several days and be easily activated to producephysiological changes. One of the major changes is acrosome exocytosis that isphysiologically related to capacitation. Glycolysis and reactive oxygen species(ROS) were studied regarding several boar sperm functions, but data available abouttheir effect on boar sperm acrosome exocytosis are scarce. The objective ofthis work was to evaluate the effect of glucose and ROS on boar sperm acrosomeexocytosis. We evaluated acrosome exocytosis by progesterone induction ofcapacitated sperm and assess viability, kinematics parameters, ROS levels, ATPcontent and Protein Kinase A activity in media with or without glucose andhydrogen peroxide or potassium chromate, as source of ROS. Our results showthat glucose has no effect on acrosome exocytosis and also, it is not necessaryfor boar sperm capacitation, although it has a positive effect in the presenceof ROS. On the other hand, ROS effects are related to spontaneous acrosomereaction. We conclude that glycolysis may function as a metabolic pathway thatprovides sustain but is not directly involved in boar sperm acrosome exocytosisand capacitation. Also, ROS do not promote capacitation in boar sperm, but affectspontaneous acrosome exocytosis.