SEIJO jose guillermo
capítulos de libros
The MAP3K-Coding QUI-GON JINN (QGJ) Gene is Essential to the Formation of Unreduced Embryo Sacs in Paspalum
MANCINI M; PERMINGEAT, HUGO; COLONO, CAROLINA; SIENA, LORENA; PUPILLI, FULVIO; AZZARO, CELESTE; DE ALENCAR DUSI, DIVA MARIA; DE CAMPOS CARNEIRO, VERA TAVARES; PODIO, MARICEL; JOSE GUILLERMO SEIJO; GONZÁLEZ, ANA MARÍA; FELITI,SILVINA; ORTIZ, JUAN PABLO A.; PESSINO, SILVINA; LEBLANC, OLIVIER
GENETICS AND GENOMICS OF PLANT REPRODUCTION FOR CROP BREEDING
Año: 2020; p. 18 - 33
Apomixis is a clonal mode of reproduction via seeds, which results from the failureof meiosis and fertilization in the sexual female reproductive pathway. In previoustranscriptomic surveys, we identified a mitogen-activated protein kinase kinase kinase(N46) displaying differential representation in florets of sexual and apomictic Paspalumnotatum genotypes. Here, we retrieved and characterized the N46 full cDNA sequencefrom sexual and apomictic floral transcriptomes. Phylogenetic analyses showed thatN46 was a member of the YODA family, which was re-named QUI-GON JINN (QGJ).Differential expression in florets of sexual and apomictic plants was confirmed by qPCR.In situ hybridization experiments revealed expression in the nucellus of aposporousplants? ovules, which was absent in sexual plants. RNAi inhibition of QGJ expressionin two apomictic genotypes resulted in significantly reduced rates of aposporousembryo sac formation, with respect to the level detected in wild type aposporousplants and transformation controls. The QGJ locus segregated independently ofapospory. However, a probe derived from a related long non-coding RNA sequence(PN_LNC_QGJ) revealed RFLP bands cosegregating with the Paspalum aposporycontrollingregion (ACR). PN_LNC_QGJ is expressed in florets of apomictic plants only.Our results indicate that the activity of QGJ in the nucellus of apomictic plants isnecessary to form non-reduced embryo sacs and that a long non-coding sequencewith regulatory potential is similar to sequences located within the ACR.