INVESTIGADORES
SEIJO Jose Guillermo
artículos
Título:
Characterization of retrotransposon sequences expressed
Autor/es:
OCHOGAVIA, A.; SEIJO, J.G; A.M. GONZÁLEZ; M. PODIO; SILVERIRA, E.; MACHADO LACERDA, A.; TAVARES DE CAMPOS CARNEIRO, V; J.P.A. ORTIZ; S.C. PESSINO
Revista:
SEXUAL PLANT REPRODUCTION
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2011 vol. 23 p. 231 - 246
ISSN:
0934-0882
Resumen:
Apomixis, an asexual mode of reproduction
through seeds, holds much promise for agricultural
advances. However, the molecular mechanisms underlying
this trait are still poorly understood. We previously isolated
several transcripts representing novel sequences differentially
expressed in reproductive tissues of sexual and apomictic
plants. Here, we report the characterization of two of
these unknown RNA transcripts (experimental codes N17
and N22). Since original fragments showed no significant
homologies to sequences at databases, preliminary genomic
PCR experiments were carried out to discard possible
contaminations. RACE extension on flanking regions provided
longer sequences for the candidates and additional
related transcripts, which revealed similarity to LTR retrotransposons
carrying short transduplicated segments of
protein-coding genes. Interestingly, some transduplicated
segments corresponded to genes previously associated with
apomictic development. Gene copy number estimations
revealed a moderate representation of the elements in the
genome, with significantly increased numbers in a sexual
genotype with respect to an apomictic one. Genetic mapping
of N17 showed that a copy of this particular element
was located onto Paspalum notatum linkage group F3c, at a
central non-recombinant region resembling a centromere.
Expression analysis showed an increased activity of N17
and N22 sense strands in ovules of the sexual genotypes. A
retrotransposon-specific differential display analysis aimed
at detecting related sequences allowed the identification of
a complex family, with the majority of its members represented
in the sexual genotype. Our results suggest that
these elements could be participating in regulatory pathways
related to apomixis and sexuality.Paspalum notatum linkage group F3c, at a
central non-recombinant region resembling a centromere.
Expression analysis showed an increased activity of N17
and N22 sense strands in ovules of the sexual genotypes. A
retrotransposon-specific differential display analysis aimed
at detecting related sequences allowed the identification of
a complex family, with the majority of its members represented
in the sexual genotype. Our results suggest that
these elements could be participating in regulatory pathways
related to apomixis and sexuality.