INVESTIGADORES
SEIJO jose guillermo
artículos
Título:
Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut.
Autor/es:
ROBLEDO, GERMÁN; LAVIA, G.I; SEIJO, J.G
Revista:
GENETICS AND MOLECULAR BIOLOGY
Editorial:
SOC BRASIL GENETICA
Referencias:
Año: 2010 p. 714 - 718
ISSN:
1415-4757
Resumen:
The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and
rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the
hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the
karyotype lacked the small A chromosomes characteristic of the A genome. In agreement with this, chromosomal
banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes.
FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes.
Collectively, these results suggest that A. trinitensis should be removed from the A genome and be considered as a B
or non-A genome species. The pattern of heterochromatic bands and rDNA loci of A. trinitensis differ markedly from
any of the complements of A. hypogaea, suggesting that the former species is unlikely to be one of the wild diploid
progenitors of the latter.