INVESTIGADORES
GARCIA Daniel Asmed
artículos
Título:
Studies with neuronal cells: from basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity.
Autor/es:
SUÑOL C; BABOT Z.; FONFRIA E; GALOFRE M; GARCIA DA; HERRERA N; IRAOLA S; VENDRELL I
Revista:
TOXICOLOGY IN VITRO : AN INTERNATIONAL JOURNAL PUBLISHED IN ASSOCIATION WITH BIBRA.
Editorial:
Elsevier
Referencias:
Año: 2008 vol. 22 p. 1350 - 1355
ISSN:
0887-2333
Resumen:
Neurotoxicology considers that chemicals perturb neurological functions by interfering with the structure
or function of neural pathways, circuits and systems. Using in vitro methods for neurotoxicity studies
should include evaluation of specific targets for the functionalism of the nervous system and general cellular
targets. In this review we present the neuronal characteristics of primary cultures of cortical neurons
and of cerebellar granule cells and their use in neurotoxicity studies. Primary cultures of cortical
neurons are constituted by around 40% of GABAergic neurons, whereas primary cultures of cerebellar
granule cells are mainly constituted by glutamatergic neurons. Both cultures express functional GABAAin vitro methods for neurotoxicity studies
should include evaluation of specific targets for the functionalism of the nervous system and general cellular
targets. In this review we present the neuronal characteristics of primary cultures of cortical neurons
and of cerebellar granule cells and their use in neurotoxicity studies. Primary cultures of cortical
neurons are constituted by around 40% of GABAergic neurons, whereas primary cultures of cerebellar
granule cells are mainly constituted by glutamatergic neurons. Both cultures express functional GABAAA
and ionotropic glutamate receptors. We present neurotoxicity studies performed in these cell cultures,
where specific neural targets related to GABA and glutamate neurotransmission are evaluated. The effects
of convulsant polychlorocycloalkane pesticides on the GABAA, glycine and NMDA receptors points to the
GABAA receptor as the neural target that accounts for their in vivo acute toxicity, whereas NMDA disturbance
might be relevant for long-term toxicity. Several compounds from a list of reference compounds,
whose severe human poisoning result in convulsions, inhibited the GABAA receptor. We also present cell
proteomic studies showing that the neurotoxic contaminant methylmercury affect mitochondrial proteins.
We conclude that the in vitro assays that have been developed can be useful for their inclusion
in an in vitro test battery to predict human toxicity.A, glycine and NMDA receptors points to the
GABAA receptor as the neural target that accounts for their in vivo acute toxicity, whereas NMDA disturbance
might be relevant for long-term toxicity. Several compounds from a list of reference compounds,
whose severe human poisoning result in convulsions, inhibited the GABAA receptor. We also present cell
proteomic studies showing that the neurotoxic contaminant methylmercury affect mitochondrial proteins.
We conclude that the in vitro assays that have been developed can be useful for their inclusion
in an in vitro test battery to predict human toxicity.A receptor as the neural target that accounts for their in vivo acute toxicity, whereas NMDA disturbance
might be relevant for long-term toxicity. Several compounds from a list of reference compounds,
whose severe human poisoning result in convulsions, inhibited the GABAA receptor. We also present cell
proteomic studies showing that the neurotoxic contaminant methylmercury affect mitochondrial proteins.
We conclude that the in vitro assays that have been developed can be useful for their inclusion
in an in vitro test battery to predict human toxicity.A receptor. We also present cell
proteomic studies showing that the neurotoxic contaminant methylmercury affect mitochondrial proteins.
We conclude that the in vitro assays that have been developed can be useful for their inclusion
in an in vitro test battery to predict human toxicity.in vitro assays that have been developed can be useful for their inclusion
in an in vitro test battery to predict human toxicity.in vitro test battery to predict human toxicity.