Isolation, identification and characterization of allelochemicals/natural products

SAMPIETRO, D.A.; CATALÁN, C. A. N.; VATTUONE, M.A.; NARWAL, S. S. (SERIES EDITOR)

Science Publishers, Inc.

Lugar: Enfield, USA; Año: 2009 p. 560

978-1-57808-577-4

The discovery of new bioactive compounds from natural products/secondary metabolites has played a major role in the development of organic chemistry. The ecological role of these allelochemicals as herbicides, insecticides and fungicides for ecological/organic sustainable agriculture has recently drawn great attention, due to increasing public concern against synthetic pesticides. Current researches on natural products emphasize not only the need of structural elucidation of the isolated compounds but also of their natural biological function and possible utility for human purposes. These concerns require reliable protocols for such studies, which sometimes are not available worldwide. This book has been written to provide information about all aspects of Allelochemicals/Natural Products. The book is divided in 3 sections - Sample Collection, Handling and Storage; Isolation, Identification and Structural Elucidation and Biological Activity of Natural Products. This book will serve as ready reference in the laboratory or class room to undergraduate and graduate students and Organic Chemists for Isolation, Identification and Characterization of Allelochemicals/Natural Products. The users of this book can select suitable methods, according to the available facilities. CONTENTS. SECTION I. Sample Collection, Handling and Storage:Allelopathy and Allelochemicals, Soil, Air and Water Samples, Plant Sampling and Sample Preparation. SECTION II. Isolation, Identification and Structural Elucidation: Colorimetric Reactions, TLC and PC, HPLC and LC-MS, Chromatography and Spectroscopy of Alkaloids, GC and GC-MS of Terpenoids, GC and GC-MS for Non-volatile Compounds, Spectrometry: Ultraviolet and Visible Spectra, Spectrometry: Infrared Spectra,NMR.SECTION III. Biological Activity of Natural Products:Bioassays with Whole Plants and Plant Organs, Bioassays on Plants: Plant Cells and Organelles, Bioassays on Microorganisms: Antifungal and Antibacterial Activities, Bioassays for Antioxidant, Genotoxic, Mutagenic, and Cytotoxic Activities, Bioassays: Inhibitors of Insect Chitin-degrading Enzymes, Bioassays: Insect Behaviour, Development and Survival, SECTION IV. Appendices: (i) Abbreviations; (ii) Chemical Formulae and Molecular Weight of Solvents and Reagents; (iii) Molecular Weight of Organic Compounds.