Expression of recombinant dengue virus type 1 non-structural protein 1 in mammalian cells and preliminary assessment of its suitability to detect human IgG antibodies elicited by viral infection

MALNERO, CRISTIAN MIGUEL; AZEVEDO, RENATA CAMPOS; BERGMANN, INGRID EVELYN; DE MENESES, MARCELO DAMIÃO FERREIRA; CAVALCANTI, ANDREA CONY; IBAÑEZ, LORENA ITATÍ*; MALIRAT, VIVIANA* *IGUAL CONTRIBUCIÓN

JOURNAL OF IMMUNOLOGICAL METHODS

ELSEVIER SCIENCE BV

Año: 2023 vol. 518

0022-1759

In recent years dengue has become a rapidly growing public health problem worldwide, however, the availability of accurate and affordable diagnostic immunoassays is limited, partly due to the difficulty of producing large quantities of purified antigen. Non-structural protein 1 (NS1) has shown to be a good candidate for inclusion in diagnostic assays and for serosurveys, particularly in endemic countries as a prerequisite for vaccination. In this work the NS1 antigen derived from dengue virus type-1 (DENV1) was expressed in HEK293-T cells and purified by affinity chromatography. The recombinant protein was recovered properly folded as dimers, highly purified and with good yield (1.5 mg/L). It was applied as a serological probe in an indirect ELISA developed in this work to detect human IgG antibodies. Preliminary comparative performance values of 81.1% sensitivity and 83.0% specificity of the developed and preliminary validated iELISA, relative to a commercial kit were obtained, suggesting that the purified recombinant DENV1 NS1 antigen is suitable to detect IgG antibodies, indicative of past DENV infection.