Unbiased Molar Mass Distributions of Narrow Polymers by Size Exclusion Chromatography with Light Scattering Detection

VEGA, J. R.; CLEMENTI, L.; MEIRA, G. R.; YOSSEN, M.; GUGLIOTTA, L. M.

Torun

Conferencia; 29th. International Symposium on Chromatography (ISC?2012); 2012

European Society for Separation Science

Current research in size exclusion chromatography (SEC) aims at developing robust and automatic correction procedures that do not require the analyst intervention. Band broadening (BB) can strongly distort the molar mass distribution (MMD) of narrow polymers when estimated by SEC. Even though several BB-correction methods have been proposed [1], they are mostly impractical, since they typically require a deconvolution procedure and the a priori estimation of the BB function (BBF) [2, 3]. For SEC with a differential refractometer (DR) and a light scattering detector (LS), this work presents a method for calculating the unbiased MMD of narrow polymers, without explicit knowledge of the BBF.