Instrumental Broadening Correction in Size Exclusion Chromatography through Fast Fourier Transform Techniques

ALBA, D.; MEIRA, G. R.

JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHNOLOGIES

Año: 1983 vol. 6 p. 2411 - 2431

1082-6076

This paper shows the practicability of the use of the fast Fourier transfora (FFT), with appropriate filtering in the fre­quency domain as a means of dcconvoluting Tung's integral formula (1). The method is limited to uniform instrumental spreading func­tions, but presents several important advantages: it is numeri­cally efficient, no assumptions about the shape of the spreading function are made, it eliminates the high-frequency measurement noise components from the corrected chromatogram without modifying the original data, and provides a means of physically interpreting the results.