Enzymatic Degradation of LigninPhenol-Formaldehyde Resins by Pseudomonas Aeruginosa

MARIANA BERNARD; VERÓNICA NICOLAU; MARISA SPONTÓN; DIANA ESTENOZ

Workshop; II Workshop on Biodegradable Polymers and Biocomposites and III Workshop BIOPURFIL; 2015

In this work the biodegradation of partially modified resols with 10% w/w of sodiumlignosulfonate and 10 and 20 % w/w of Kraft lignin type is studied. The experimental work involved preliminary studies of biodegradation in Petri dish (clear zones), the degradation of resols by enzymatic attack of Pseudomonas aeruginosa under aerobic conditions for a period of 300 days and the characterization of the polymers before and afterbiodegradation by gas chromatography (GC) The number of viable cells showed a significant increase during the process. However, the gravimetric analysis was not sufficient to check the biodegradation. The results indicated that endocellular enzymes could be involved. It was observed that the presence of low concentrations of toxic substances released during degradation of the material may have inhibitory effects. Resoles were synthesized in Centro S.A., San Francisco, Córdoba