Post-harvest sugarcane residue degradation by autochthonous fungi

MARIANELA MAZA; HIPÓLITO F. PAJOT; MARÍA JULIA AMOROSO; MARTA GRACIELA YASEM

INTERNATIONAL BIODETERIORATION & BIODEGRADATION

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2014 vol. 87 p. 18 - 25

0964-8305

Several fungal species were isolated from different sources: post-harvest sugarcane residue, soil,decomposing forest litter and from mycelia obtained from the inner parts of fresh fungal fruiting bodiescollected in Las Yungas region (Argentina). These isolates werefirst screened for their ability to producecarboxymethyl cellulose (CMC) degradation and guaiacol oxidation. After primary screening, seventeenisolates were further tested for their ligninolytic ability by assessing polyphenoloxidase, laccase, manganese peroxidase and endoxylanase activities. Based on their lignocellulolytic activities,five isolates(namedBjerkanderasp. Y-HHM2,Phanerochaetesp. Y-RN1,Pleurotussp. Y-RN3,Hypocrea nigricansSCT-4.4 andMyrotheciumsp. S-3.20) were selected for liquid and solid-state fermentation assays in culturemedia including sugarcane debris. Lignocellulolytic enzymes production, dry mass loss and phenolconcentration in the water soluble fraction were then evaluated. Results suggest that native strains withlignocellulolytic activity are suitable to increase post-harvest sugarcane residue decomposition andsupport the use of these strains as an alternative to pre and post-harvest burning. Biological treatmentsusingPhanerochaetesp. Y-RN1,Pleurotussp. Y-RN3 andMyrotheciumsp. S-3.20 could be used to degradeand increase the accessibility to lignocellulose components of sugarcane residue.