High collinear three-way photoinduced spectrofluorimetric data arrays modelled with bilinear least-squares: determination of tetracyclines in surface water samples

GOICOECHEA, HÉCTOR C; GIL GARCÍA, MARÍA; MARTINEZ GALERA, MARIA; SANTIAGO VALVERDE, ROSARIO

Lugo

Congreso; X International Symposium on Luminiscence Spectrometry - Detection Techniques in Biomedical, Environmental and Food Analysis; 2006

SLS

A new sensitive method for the determination of three tetracyclines in surface water samples (tetracycline, chlortetracycline and oxytetracycline) using highly collinear data corresponding to photochemical induced fluorescence excitation emission matrices, modelled with three-way data arrays chemometric tools has been developed. The method involves irradiation of aqueous basic solution of antibiotics with UV light and fluorescence determination of the generated photoproducts after SPE with Oasis HLB cartridges. The chemometric models were built by using an 8-samples calibration set and samples corresponding to both a validation set and a test set. The latter set containing unsuspected interferents.  Considering this latter samples, recoveries of 101.5, 97.9 and 102.2 were obtained for the three analytes. Evaluation of constant and proportional bias and precision was made by using the elliptical joint confidence region (EJCR) test for the estimated intercept and slope