Polylactic acid sheets seeded with genetically modified ovine diaphragmatic myoblasts for myocardial regeneration. Preliminary results

C.S. GIMENEZ; F.D. OLEA; P. LOCATELLI; M.R. BAUZA; R. DEWEY; F. MONTINI BALLARIN; G.A. ABRAHAM; A. ORLOWSKI; A. DE LORENZI; L. CUNIBERTI; A. CROTTOGINI

Mar del Plata

Otro; LXI Reunión científica anual de la Sociedad Argentina de Investigación Clínica, SAIC-SAI-SAFE 2016; 2016

SAIC

We tested the hypothesis that polylactic acid (PLA) sheets seeded with diaphragmatic myoblasts (DM) overexpressing connexin-43 (Cx43) would decrease infarct size and improve left ventricular (LV) function in sheep with chronic myocardial infarction (MI). Forty minutes after coronary artery ligation, PLA sheets without DM (PLA, control group, n=4) or PLA sheets with DM overexpressing Cx43 (PLA-DM-Cx43 group, n=4) were sutured covering the infarcted zone and 1 cm of the adjacent peri-infarct zone. Baseline echocardiograms confirmed that all animals displayed normal LV function prior to infarction. Cardiac magnetic resonance with gadolinium enhancement was performed at 3 and 45 days after MI to determine infarct size and LV function early and late after treatment, respectively. Blinded data analysis revealed that infarct size expressed as ml of infarcted tissue decreased in the PLA-DM-Cx43 group from 8.0±1.83 ml at day 3 to 6.25 ±2.2 ml at day 45 (p