Silver nanoparticles synthesized with quercetin: synthesis, characterization and effect in human trophoblast exposed to neonicotinoids

BUSTOS, PAMELA SOLEDAD; LOPEZ VENDITTI, ELIANA; PAEZ PAULINA; ORTEGA MARIA GABRIELA; GUIÑAZU ALANIZ, NATALIA LORENA

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigacion Clínica (SAIC) - Reunión Anual de Sociedades de Biociencias 2019; 2019

Sociedad Argentina de Investigacion Clinica

Silver nanoparticles gained popularity due to their applications in medicine. The synthesis of nanoparticles using plant extracts or their antioxidant metabolites is the most adopted method for green production. Previous studies showed toxic effects of the insecticide neonicotinoid (Neo) acetamiprid on human trophoblasts mainly due to oxidative imbalance. The aim of this work was to obtain green silver nanoparticles (AgNPs) able to avoid Neo toxicity. AgNPs were synthesized in the presence of a novel plan antioxidant quercetin (Q), a flavonoid isolated from Flaveria bidentis.AgNPs synthesis conditions: AgNO3 (1-10 mM), Q (0.5-2 mM), sodium citrate as stabilizer, stirring time (0-24 h) and pH (7-12). AgNPs were characterized by UV/Vis spectroscopy, TEM and dynamic light scattering. To test the possible protective effects the HTR-8/SVneo trophoblast cell line was pre-treated 1 h with AgNPs, and then incubated for 24 h with Neo (100 µM). Controls of synthesis precursors, Neo and AgNPs dilutions (1/5-1/1000) were included. Three AgNPs were obtained, AgQ3NPs (18 nm), AgQC4NPs (50 nm) and AgQC7NPs (33 nm). AgQC7NPs, unlike its precursors, showed no cytotoxic effect on trophoblast, while AgQ3NPs and AgQC4NPs caused a significant decrease in cell viability at 1/5 dilution. Regarding the protective AgNPs effect against Neo toxicity, AgQ3NPs and AgQC7NPs at 1/250, 1/500 and 1/1000 dilutions, avoided the decrease of cell viability (MTT method) and the reative oxygen species (ROS) production (NBT method) induced by Neo. AgQC4NPs, avoided cell viability loss at 1/500 and 1/1000 dilutions, with a concentration-dependent effect ROS production reaching values significantly similar to the control at 1/1000. Green Q synthesized AgNPs demonstrated a protective effect for Neo induced cytotoxicity and oxidative imbalance in trophoblast. Further studies will be necessary to determine the mechanisms responsible for these effects.