Spleen B cells from BALB/c are more prone to activation than spleen B cells from C57BL/6 mice during a secondary immune response to cruzipain

ANDREA PELLEGRINI; NATALIA GUIÑAZÚ; MARI PILAR AOKI; ISABEL CHICO CALERO; EUGENIO ANTONIO CARRERA-SILVA; NURIA GIRONES; MANUEL FRESNO; SUSANA GEA

INTERNATIONAL IMMUNOLOGY

Oxford University Press

Lugar: Washington DC; Año: 2007 vol. 19 p. 1395 - 1402

0953-8178

There is an increasing interest in the study of roles that B cells may play in regulating immune responses both in protection and in pathogenesis. However, little is known about additional immune functions of B cells independently of antibody production. In this study, we have assessed how the immunization with T-dependent antigens in different host genetic backgrounds affects several parameters of B cells during secondary immune responses. We have previously reported that BALB/c immunized with cruzipain, induced heart autoimmunity, whereas C57BL/6 mice were resistant. In a comparative study employing the same experimental model, we demonstrated that BALB/c-enriched spleen B cells presented higher ability to proliferate releasing elevated levels of IL-4. Moreover, spleen of immune BALB/c mice presented an increased number of germinal center and plasma cells as well as higher expression of B-cell activation markers (MHC class II, CD40, CD86). These findings demonstrate the influence of genetic background on B-cell activation and emphasize the importance of examining B-cell behavior in the context of the specific immunogens.