Lactic bacteria of goats during shift from forage to concentrate diet.

GRILLI, D; FLIEGEROVA K; KOPECNY, J; SOHAEFER N; PEREYRA C; RUIZ S; QUIROGA L; VERNOLA, S; SOSA, MA; ARENAS, GN; MRAZEK, J

Tucumán

Simposio; V International Symposium on Lactic Acid Bacteria; 2016

Centro de Referencia para Lactobacilos (CERELA) , Tucumán

High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. In adult sheep and cattle, Streptococcus bovis and lactobacilli can increase in ruminants being fed or being switched to high-grain diet. This study was therefore aimed to evaluate the dynamics of Lactobacillus/Streptococcus group and others ruminal bacteria of 4 ruminally cannulated goats during the switching from a forage diet to a concentrate diet by retrieving partial 16S rDNA sequences analyzed by quantitative polymerase chain reaction (qPCR) and Next Generation Sequencing (NGS) approach. The animals were fed on alfalfa hay (AH) diet for a period of 30 days. Then goats were abruptly shifted to a mixed forage - concentrate diet consisting of 60% alfalfa hay and 40% corn grain (AH/C diet) for 20 days. The ruminal contents were sampled from each goat on day 2, 10, and 20 of the experimental period. The dietary transition of goats from AH- to AH/C-diet resulted in the significant decrease of rumen fluid pH. The significantly (P