Initial steps for a microbiologic study of rumen in small ruminant.

GRILLI, D; DEIS, E.; LAUSI, O; DUARTE, A.; FLORES, D.; DEGARBO, S.; CABALLERO, P.; TELECHEA, A.; ARUANI, P; SMACCHIA, A.; FERNÁNDEZ, R.; ARENAS, GN.; FAIN BINDA, J.

Cordoba

Jornada; XXV Reunión anual de la Sociedad de Biología de Cuyo; 2007

We began a microbiologic study about microbiotic ruminal in small ruminant of Mendoza’ zoo. We used adult Somali’s sheep and goats. The animals were previously vaccinated against mancha, gangrene and tetanus and were treated with antibiotics and antihelmintics. The animal were uninformed 24 hours of food and 12 hours of liquid. Then, we made the surgery in Veterinary School of Maza University. This procedure is a rumenotomy, with application of intraruminal cannula. This dispositive was made with PVC (polivinilcarbonate), sealed in the soft parts with rubber rings, that prevents the ebb tide. The animals were placed in a closed and clean place, for special cares, mainly for disinfection of the injury until healing and final adaptation of the cannula. A chronic no inflammatory fistula, with an animal of normal behavior, allows us to follow the next step: the application of an own design dispositive to take aseptic samples, based in hydraulics forces. We compared thus results obtained with other procedures. The first isolation was made in a Tarozzi agar and 4% agar with an anaerobic atmosphere. Colonies will be picked in a slob tube with 2% agar. Then, we differentiated aerobic from anaerobic bacterium and made biochemical tests and the quantification of organic acid fermentation products, established by gaseous chromatography. We also prepared RCGA (40% ruminal fluid, glucose, cellobiose, agar) medium, in which the ruminal fluids were included in a 1:3 proportion in medium, to replace the nutrients that originated the own ruminal bacterium. We will also use Med 10 medium, prepared with a mixture of volatile fatty acids, without ruminal fluid. Ketorolac (0,3mg/kg/day) was used after surgery to handle the pain for a week, with the best recovery.