PERSONAL DE APOYO
DE GENARO pablo Adrian
congresos y reuniones científicas
Título:
Retinoic Acid stimulates the differentiation of retina photoreceptors in vitro
Autor/es:
DE GENARO, P; ROTSTEIN, NP; POLITI, LE
Reunión:
Congreso; Segunda Reunion Conjunta de Neurociencias; 2010
Resumen:
Retinoic Acid
(RA), a vitamin A prime metabolite, is crucial for correct eye development and
photoreceptor (PR) differentiation. We here investigated its effects on early
PR development and the intracellular pathways involved. Pure neuronal cultures
obtained from 2 day old rat retinae were suplemented with or without 10 nM RA a
few hours after plating. RA almost doubled the amount of cells expressing opsin
and stimulated the expression of peripherin, a disc structural component, and
the formation of apical processes in PRs at every time studied. We then assessed
the activation of p38-MAPK, which is
involved in RA receptor phosphorylation; RA readily increased phosphorylated p38 levels whereas total p38 remained unchanged. Addition of SB203580,
a p38 inhibitor, led to a partial
reversion of RA-stimulated opsin expression. We next studied the participation
of PI3K, which is thought to have opposite effects to those of p38. Opsin expression was higher in
cultures treated with RA plus Wortmannin or LY294002, PI3K inhibitors, than in
those only with RA, suggesting RA might negatively regulate PI3K.
In summary
this work shows that RA promotes early development in vitro of PRs and this stimulation may occur, at least in part,
through p38-MAPK activation and
negative regulation of PI3K.