1α,25(OH) 2 D 3 -glycosides from Solanum glaucophyllum leaves extract induce myoblasts differentiation through p38 MAPK and AKT activation

IRAZOQUI, ANA PAULA; DE GENARO, PABLO; BUITRAGO, CLAUDIA; BACHMANN, HEINRICH; GONZÁLEZ-PARDO, VERÓNICA; RUSSO DE BOLAND, ANA

Biology Open

The Compay of Biologists

Año: 2018 vol. 7

We have previously shown that Solanum glaucophyllum leaf extract(SGE) increases VDR protein levels and promotes myoblastdifferentiation. Here, we investigated whether p38 MAPK and AKTare involved in SGE actions. Cell-cycle studies showed that SGEprompted a peak of S-phase followed by an arrest in the G0/G1-phasethrough p38 MAPK. Time course studies showed that p38 MAPK andAKT phosphorylation were statistically increased by SGE (10 nM) orsynthetic 1α,25(OH)2D3 (1 nM) treatment. Furthermore, p38 MAPKand AKT inhibitors, SB203580 and LY294002 respectively,suppressed myoblasts fusion induced by SGE or synthetic1α,25(OH)2D3. We have also studied differentiation genes by qRTPCR. myoD1 mRNA increased significantly by SGE (24?72 h) or1α,25(OH)2D3 (24 h) treatment. mRNA expression of myogenin alsoincreased upon SGE or 1α,25(OH)2D3 treatment. Finally, MHC2bmRNA expression, a late differentiation marker, was increasedsignificantly by both compounds at 72 h compared to control. Takentogether, these results suggest that SGE, as synthetic 1α,25(OH)2D3,promotes myotube formation through p38 MAPK and AKT activation