156) METABOLIC ALTERATIONS IN AMYGDALA INDUCED BY THE ACUTE ADMINISTRATION OF KETAMINE (Preliminary study).

GUEVARA, MANUEL A.; LORENZO, S.; MARTIN CUNIETTI, J.; GARCIA MENENDEZ, S,; LAFUENTE, J.V.; BAIARDI, G.; GARGIULO, P.A.

Mendoza

Simposio; IIº International Symposium of Translational Medicine. XII Annual Meeting of Investigation at the Faculty of Medical Sciences. Mendoza. 7 a 8 de marzo de 2019.-; 2019

Facultad de Ciencias Médicas. Universidad Nacional de Cuyo.

156) Guevara M, Lorenzo S, Martin Cunietti, S, García Menéndez S, Lafuente J, Baiardi G, Gargiulo PA. Metabolic alterations in amygdala induced by the acute administration of Ketamine (Preliminary study). IIº International Symposium of Translational Medicine. XII Annual Meeting of Investigation at the Faculty of Medical Sciences. Mendoza. 7 a 8 de marzo de 2019.-http://fcm.uncuyo.edu.ar/prorroga-para-cargar-resumenes-a-las-xiv-jornadas-de-investigacion-fcm-y-el-ii-simposio-de-medicina-traslacionalIntroduction and objectives: Ketamine is a general anaesthetic that acts as an antagonist of the N-methyl-D-aspartic acid (NMDA) receptors, as well as the psychotrogenic compound Phencyclidine (PCP), a drug recognized for its drug-induced psychotogenic effect. In subanesthetic doses, ketamine produces hallucinations, and for this reason it has been used as a model of schizophrenia in animals. The behavioral disorders induced by this compound in subanesthetic doses are subject of study in our laboratory.Materials and methods: In the present work we studied the effect produced by the systemic administration of ketamine at subanesthetic doses on metabolic activity in amygdala in male rats derived from the Holtzman strain (weight 240-280 gr). Three doses were systemically administered in a saline vehicle. They were compared: 1.25, 2.50 and 5 mg / kg (n = 5 per group). The metabolic activity was evaluated using MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) through the spectrophotometric measurement of the wavelength.Results: Metabolic activity showed a clear and significant decrease in the amygdala (1.25 mg / kg, p