In vivo detection of free radicals in mouse septic endephalopathy using MRI and immuno-spin trapping

RHEAL TOWNER; GARTEISER, P; BOZZA, F; SMITH, N.; D'AVILA; MANGO, F; RAMIREZ DC; SANDRA E GOMEZ-MEJIBA; MASON, RP; FARIA-NIETO, H.

FREE RADICAL BIOLOGY AND MEDICINE

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2013 vol. 65 p. 828 - 837

0891-5849

Free Radic Biol Med. 2013 Dec;65:828-37. doi: 10.1016/j.freeradbiomed.2013.08.172. Epub 2013 Aug 23. In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping. Towner RA1, Garteiser P, Bozza F, Smith N, Saunders D, d´ Avila JC, Magno F, Oliveira MF, Ehrenshaft M, Lupu F, Silasi-Mansat R, Ramirez DC, Gomez-Mejiba SE, Mason RP, Castro Faria-Neto HC. Author information 1Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. Electronic address: Rheal-Towner@omrf.org. Abstract Free radicals are known to play a major role in sepsis. Combined immuno-spin trapping and molecular magnetic resonance imaging (MRI) was used to detect in vivo and in situ levels of free radicals in murine septic encephalopathy after cecal ligation and puncture (CLP). DMPO (5,5-dimethyl pyrroline N-oxide) was injected over 6h after CLP, before administration of an anti-DMPO probe (anti-DMPO antibody bound to albumin-gadolinium-diethylene triamine pentaacetic acid-biotin MRI targeting contrast agent). In vitro assessment of the anti-DMPO probe in