DENDRITIC CELL MIGRATION TO REGIONAL LYMPH NODES CONTRIBUTES TO YERSINIA ENTEROCOLITICA O:3-INDUCED REACTIVE ARTHRITIS IN TNFRP55-/- MICE

SILVA JUAN EDUARDO; ANDREA CONSTANZA MAYORDOMO; MABEL NOEMÍ DAVE; CLAUDIA AGUILERA MERLO; ELIÇABE RICARDO JAVIER; , DI GENARO MS

San Miguel de Tucumán

Congreso; LVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2019

Sociedad Argentina de Inmunología

Background: Reactive arthritis (ReA) may develop after gastrointestinal or genitourinary infections. Dendritic cells (DCs) are central for T lymphocyte activation. We have reported that TNFRp55-/- mice develop chronic Yersinia enterocolitica (Ye)-induced ReA. On the arthritis onset (day 14), these mice presented an increase of migratory DCs (mDC:MHCIIhiCD11c+) and resident DCs (rDC:MCHIIintCD11c+) in mesenteric lymph node (MLNs) and regional lymph nodes to joints (RLN). The role of DCs in ReA is unknown. The purpose was to study the contribution of DC migration to ReA.Methods: C57BL/6 wild-type (WT) and TNFRp55-/- mice were orally infected with 1-5× 108 Ye O:3. To block DC migration, fingolimod (FTY72) (5mg/kg) was administered intraperitoneally at days 14, 17 and 20 after infection. Clinical score was calculated until day 21, and DCs from RLNs were analyzed by flow cytometry. Bone marrow-derived DCs (BMDCs) of WT or TNFRp55-/- were in vitro infected with Ye O:3, and the CCR7 expression was analyzed by flow cytometry. The IL12/23p40 and IFN-γ levels were measured by enzyme-linked immunosorbent assay (ELISA) in homogenates of the joints.Results: At day 21, FTY720-treated TNFRp55-/- mice decreased their arthritis clinical score and the absolute number of mDC and rDC (CD11b+ or CD11- subsets) in their RLNs (p