SYNOVIAL MICROENVIRONMENT OF SPONDYLOARTHRITIS IMPACTS ON FIBROBLAST-LIKE-SINOVIOCYTE: ROLE OF IL-6 AND S100A8 PROTEIN

, DI GENARO MS; ELIÇABE RICARDO JAVIER; SILVA JUAN EDUARDO; BLAS RODRIGO; MUNARRIZ ALICIA; PARDO HIDALGO R; TAMASHIRO H

San Miguel de Tucumán

Congreso; LVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2019

Sociedad Argentina de Inmunología

The molecular mechanisms driving the distinct characteristic of fibroblast-like synoviocytes (FLS) in spondyloarthritis (SpA) remain unknown. We have reported that synovial fluid (SF) of SpA patients induces interleukin (IL)-6 secretion by FLS. Furthermore, a proteomic study of SF of SpA showed the presence of the alarmin S100A8.The objective of the present work was to study the effect of the synovial S100A8 on the IL-6 production by FLS. The expression of alpha-smooth-muscle-actin (a-SMA), a miofibroblast marker, was also studied. The SF of SpA patients were pooled (n=9). Human FLS of the SW982 cell line were stimulated with the SF pool at dilution 1:10 in absence or presence of anti-S100A8/A9 antibodies. Different concentration of the S100A8 (1-10 ug/ml) were also assayed. After 24-96 h, the IL-6 levels in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA). Moreover, FLS were stained intracellularly for a-SMA and analyzed by flow cytometry. The data were analyzed by the two-way analysis of variance (ANOVA) followed by the Bonferroni posttest. A p value < 0.05 was considered statistically significant. We confirm that SpA SF induces IL-6 production by FLS (p