INFLAMATORY RESPONSE IN LUNG OF WILD TYPE AND

GUTIéRREZ JV; DI GENARO MS; GóMEZ NN

Mendoza

Congreso; XXVIII Reunion Anual de la Sociedad de Biologia de Cuyo; 2011

Sociedad de Biologia de Cuyo

Yersinia enterocolitica is a Gram-negative bacterium that affects both humans and rodents. This pathogen can spread in systemic way causing injury in liver, spleen and lung. IL-12 plays a principal role in the protective immune response. The purpose of this work was to determine if IL-12p40 deficiency would contribute with the lung injury due to weak and inappropriate immune response. C57BL/6 (WT) and C57BL/6 IL-12p40 -/- (KO) mice were orogastrically infected with 2x107- 2x108 colony-forming units (CFU) of Y. enterocolitica 0:3. Mice were sacrificed at day 14 postinfection. Uninfected mice were used as control. RT-PCR was performed from lung tissue and the mRNA expression of TLR-2, TLR-4, I-CAM, IFN-ã, TNF-á genes were measured. Histological changes in lung were also analyzed. We observed increased expression of TLR-2 (p<0.05), and I-CAM (p<0.05) in KO compared with WT mice and TLR-4 and IFN-ã increased but not significantly in KO; but TNF-á don’t change. Histophatological change reflected this situation. These results suggest that increased expression of TLR-2 and TLR-4 could lead at KO group to an exacerbated inflamatory response that in addition with increased I-CAM, which participates in lymphocytes rolling to the infection site, contribute with the increased cellular infiltration. Therefore, IL-12p40 contributes in protection against lung injury.is a Gram-negative bacterium that affects both humans and rodents. This pathogen can spread in systemic way causing injury in liver, spleen and lung. IL-12 plays a principal role in the protective immune response. The purpose of this work was to determine if IL-12p40 deficiency would contribute with the lung injury due to weak and inappropriate immune response. C57BL/6 (WT) and C57BL/6 IL-12p40 -/- (KO) mice were orogastrically infected with 2x107- 2x108 colony-forming units (CFU) of Y. enterocolitica 0:3. Mice were sacrificed at day 14 postinfection. Uninfected mice were used as control. RT-PCR was performed from lung tissue and the mRNA expression of TLR-2, TLR-4, I-CAM, IFN-ã, TNF-á genes were measured. Histological changes in lung were also analyzed. We observed increased expression of TLR-2 (p<0.05), and I-CAM (p<0.05) in KO compared with WT mice and TLR-4 and IFN-ã increased but not significantly in KO; but TNF-á don’t change. Histophatological change reflected this situation. These results suggest that increased expression of TLR-2 and TLR-4 could lead at KO group to an exacerbated inflamatory response that in addition with increased I-CAM, which participates in lymphocytes rolling to the infection site, contribute with the increased cellular infiltration. Therefore, IL-12p40 contributes in protection against lung injury.