GALLO CALDERON marina beatriz
congresos y reuniones científicas
Genome evolution of canine parvovirus in South America
RUBEN PEREZ; SOFÍA GRECCO; ANA PAULA SILVA ; JAIME ALDAZ; GALLO CALDERON, MARINA; ALICE ALFIERI; LUCÍA CALLEROS; ANA MARANDINO; GREGORIO IRAOLA; LOURDES FRANCIA; RODRIGO MARTINO; YANINA PANZERA
Congreso; Xth International Congress of Veterinary Virology; 2015
European Society for Veterinary Virology
Canine parvovirus (CPV) causes one of the most significant infectious diseases of dogs that has to be controlled by early vaccination. Its genome has a linear single-stranded DNA genome (5.2 kb) that codes for nonstructural (NS) and capsid (VP) proteins. In the present study, we analyze CPVs from South America to characterize the strains and to provide new insights into local and global viral diversity andevolution.Sampleswere collected from Uruguay, Argentina, Brazil, Paraguay and Ecuador in the last decade. The complete genome was obtained in a single PCR reaction and directly sequenced. Phylodynamics analysis was performed to characterize the circulating strains and estimate the time of the most recent common ancestor.We identified fourCPV phylogenetic groupsor circulating in South America. These groups have different evolutionary history and residence time and are unevenly distributed in South America. Two clear recombinant sequences were detected in Ecuador and Uruguay. Some relevant amino acid chances occur in positive selective sites in the VP and NS proteins. Our findings reveal that several factors contribute to the current distribution of canine parvovirus in South America. There is co-circulation and spreading of genotypes from different geographic origin, local differentiation by the acquisition of particular amino acid changes in positive selected sites, deletions in non-coding sequences (VP1 intron) and recombination. CPV variability reveals a particular dynamics in the continent that provide new insight into the evolution of this relevant virus.