A comprehensive analysis of ploidy-regulated gene expression in Paspalum notatum

MARTELOTTO, L.; ORTIZ, J. P. A.; FRANCISCO ESPINOZA; QUARIN, C. L.; PESSINO, S. C.

Puerto Iguazú (Mnes)

Congreso; XL Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2004

PL-C4. A COMPREHENSIVE ANALYSIS OF PLOIDYREGULATED GENE EXPRESSION IN Paspalum notatum Paspalum notatum Martelotto LG, Ortiz JPA, Espinoza F, Quarin CL, Pessino SC. Laboratorio Central de Investigaciones, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, 2123 Zavalla, Santa Fe; IBONE-CONICET, FCA-UNNE, 3400 Corrientes. E-mail: lubio77@hotmail.com Although the causes of novel variation in polyploids are not well understood, they could involve changes in gene expression through dosage-regulation and altered regulatory interactions, as well as rapid genetic and epigenetic changes. The wide-genome expression of ~ 10000 genes was monitored by using transcript profiling technology on flowers of a diploid genotype and its newly-formed autotetraploid derivative. Eighty total primer pair combinations were assayed in differential display experiments. Out of 129 polymorphic bands, 90 were succesfully reamplified and cloned. Differential expression of 64 clones was validated by reverse Northern blot. Sequence analysis showed that 42 clones corresponded to 26 distinct genes of known function (E values < e-4) while the remaining 22 were classified as novel sequences. Genes detected were involved in processes of recombination, chromatine structure modification, regulation of transcription, proteolisis and its regulation, protein folding, carbohydrate metabolism and signal transduction. Curiously, two highlyadenylated mitochondrial-encoded genes were found to be differentially regulated. The genetic structure of both lines, examined with 565 RAPD markers, revealed 9.2% of polymorphisms.-4) while the remaining 22 were classified as novel sequences. Genes detected were involved in processes of recombination, chromatine structure odification, regulation of transcription, proteolisis and its regulation, protein folding, carbohydrate metabolism and signal transduction. Curiously, two highlyadenylated mitochondrial-encoded genes were found to be differentially regulated. The genetic structure of both lines, examined with 565 APD markers, revealed 9.2% of polymorphisms. -4) while the remaining 22 were classified as novel sequences. Genes detected were involved in processes of recombination, chromatine structure odification, regulation of transcription, proteolisis and its regulation, protein folding, carbohydrate metabolism and signal transduction. Curiously, two highlyadenylated mitochondrial-encoded genes were found to be differentially regulated. The genetic structure of both lines, examined with 565 APD markers, revealed 9.2% of polymorphisms.