INVESTIGADORES
FRANCHI Ana Maria
artículos
Título:
Ethanol downregulates N-acylphosphatidylethanolamine- phospholipase DexpressioninBV2microglialcellsviaepigenetic mechanisms
Autor/es:
CORREA FERNANDO GABRIEL; A DE LAURENTIIS; FRANCHI ANA MARIA
Revista:
EUROPEAN JOURNAL OF PHARMACOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 786 p. 224 - 233
ISSN:
0014-2999
Resumen:
Excessiveethanoldrinkinghasdeleteriouseffectsonthebrain.However,theeffectsofalcoholonmi-croglia, themainmediatorofthebrain'sinnateimmuneresponseremainpoorlyunderstood.Ontheother hand,theendocannabinoidsystemplaysafundamentalroleinregulatingmicroglialreactivityandfunction. HerewestudiedtheeffectsofacuteethanolexposuretomurineBV2microglialcellson N-acylphosphatidylethanolamine-phospholipase D(NAPE-PLD),amajorsynthesizingenzymeofanandamideand other N-acylethanolamines. WefoundthatethanoldownregulatedmicroglialNAPE-PLDexpressionby activatingcAMP/PKAandERK1/2.Thesesignalingpathwaysconvergedonincreasedphosphorylationof CREB.Moreover,ethanolinducedandincreaseinhistoneacetyltransferaseactivitywhichledtohigherlevels ofacetylationofhistoneH3.Takentogether,ourresultssuggestthatethanolactionsonmicroglialNAPE-PLDexpressionmightinvolveepigeneticmechanisms.
