The nitric oxide synthase of mouse spermatozoa

HERRERO MARIA BELEN; GOIN JUAN CARLOS; BOQUET MARIEL; CANTEROS MARIA GRISELDA; FRANCHI ANA MARIA; PEREZ MARTINEZ SILVINA; POLAK JULIA; VIGGIANO MARCELO; GIMENO MARTA

FEBS LETTERS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1998 vol. 411 p. 39 - 42

0014-5793

Nitric oxide synthase (NOS) was evidenced in mature mouse spermatozoa by means of biochemical techniques and Western blot. During 120 min of incubation, 10(7) spermatozoa synthesized 7 +/- 2 pmol of L-[14C]citrulline. Besides, L-citrulline formation depended on the incubation time and on the concentration of L-arginine present in the incubation medium. Different concentrations of N(G)-nitro-L-arginine methyl ester (L-NAME) but not aminoguanidine, inhibited L-[14C]citrulline formation. Western-blot analysis of solubilized sperm proteins revealed a unique band of M(r)=140 kDa with the neural, endothelial and inducible NOS antisera tested. These results provide evidence that mature mouse sperm contains a NOS isoform and that spermatozoa have the potential ability to synthesize NO, suggesting a role for endogenous NO on mammalian sperm function.