INVESTIGADORES
FONSECA Maria isabel
artículos
Título:
Fungal species originating from chromium contaminated soil for ecofriendly and biotechnological processes
Autor/es:
TATARIN, ANA SILVIA; ARANGUIZ, CAMILA; SADAÑOSKI, MARCELA ALEJANDRA; POLTI, MARTA ALEJANDRA; FONSECA, MARÍA ISABEL
Revista:
APPLIED SOIL ECOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2023
ISSN:
0929-1393
Resumen:
Chromium contamination through different anthropogenic activities has been of great concern in soil health dueto its toxicity and persistence. Microbial originating from contaminated industrial soil, including chromiumtolerant fungi, is a promising approach to adapt green technologies. The current study investigated chromiumcontaminated industrial soils to explore the potential of indigenous fungi to tolerate, remove and detoxify high Cr(VI) contaminated matrices, as well as, the employed mechanisms by the most promising strain. This study involvesfungal isolation from a chromium contaminated soil, selection and identification using ITS1 and tef1sequences of the most chromium tolerant fungal strain. Mycoremediation was assessed by Cr(VI) removal anddetoxification employing Lactuca sativa and Artemia salina tests, whereas scanning electron microscopy (SEM),energy dispersive X-ray (EDX) and chromate reductase activity were evaluated to understand the implicatedmechanisms. Isolation of 12 distinct fungal strains was carried out classified into three genera: Trichoderma sp.,Penicillium sp., and Aspergillus sp. The identified strain as Trichoderma koningiopsis LBM 253 demonstrated thehighest tolerance in solid medium and also removed up of the metal in liquid media at an high initial Cr(VI)concentration of 200 mg L􀀀 1. Fungus showed the ability to bioaccumulate Cr(VI) and SEM and EDX analysisshowed that the adsorbed chromium was sequestered in the fungal mycelium as a precipitate. Moreover, hightiters of chromate reductase activity were detected, and changes were observed in the extracellular and intracellularactivity. Ecotoxicity tests carried out using plant (L. sativa) and animal (A. salina) biomarkers showedthat T. koningiopsis decreases Cr(VI) toxicity in liquid systems. The findings of this study provide strong evidencethat T. koningiopsis LBM 253 originated from chromium-contaminated soil is a promising fungus to be applied inmycoremediation and ecofriendly processes, offering the capability to tolerate, remove, and detoxify highconcentrations of Cr(VI) from liquid media supplemented with the metal.