INVESTIGADORES
FIDELIO gerardo Daniel
congresos y reuniones científicas
Título:
Surface Properties of amphiphilic peptides
Autor/es:
FIDELIO G.D.
Lugar:
Bilbao
Reunión:
Conferencia; BioForo; 2014
Institución organizadora:
Universidad Pais Vasco, Facultad de Ciencia y Tecnología, Unidad de Biofisica
Resumen:
Modern view of natural membranes has been updated
from the original vision emerged from the Fluid Mosaic Model of Singer and
Nicolson published in 1972. One of highlighted feature of the new vision is the acceptance of a high density of proteins mixed with lipids organized as a
bilayer forming a continuous hydrophobic/hydrophilic limit that dynamically
controls the function of biomembranes in the cell. Langmuir monolayers at the
air/water interface is practically the unique technique allowing the study of
the surface properties of peptides and proteins and their interaction with
lipids in a confined ?crowded? condition similar to the that found in
natural biomembranes. Even more, the experimental conditions, as the surface
covered proportion of the peptide compared with the lipid one or the degree of
surface compactness, are quite controlled at will by the investigator. Using
Langmuir monolayer many studies can be performed such us: ability of peptides
to absorb to clean air/water interfaces (tensio-active properties), to interact with organized lipid monolayers
(penetration), surface stability of spread protein/peptide sample,
peptide/lipid lateral miscibility, surface rheology, conformation and secondary
structure by PM-IRRAS and lateral topography by using the Brewster Angle Microscopy technique (BAM).