Chromosomal aberrations induced by Mitomycin C in canine lymphocytes

CALIRI M; D. M. FERRÉ,; LEDDA VALERIA; N GORLA,

VETERINARSKI ARHIV

UNIV ZAGREB VET FACULTY

Lugar: Zagreb; Año: 2023 vol. 93 p. 341 - 350

0372-5480

Chromosomal aberrations comprise an abnormal number of chromosomes as well as changes in the structure of thechromosomes. Aberrations in the structure of chromosomes, such as gaps and breaks in chromatids and chromosomes,acentric fragments, telomeric associations, deletions, early chromatid-separation and large scale effects, such aspulverized metaphases, and sticky metaphases. In this study, the effect of Mitomycin C, a recognized clastogenon human lymphocytes, was assayed in cultures of canine lymphocytes, an animal species that is flourishing incomparative medicine. Blood cultures were performed on samples from a male and a female dog. Cytotoxicity usingthe mitotic index and genotoxicity testing were performed with 0.25 µg/ml Mitomycin C. The total chromosomalaberrations were significantly higher due to the effect of Mitomycin C (P=0.0247). The number of chromatid breaksnearly quintupled, while pulverized metaphases were found to be six times more frequent, and endoduplicated cellswere three times higher than in negative control cultures. The quantitatively most relevant chromosomal aberrationwas the presence of sticky metaphases, related to adverse effects in chromatin proteins. The potential use of caninelymphocytes for chromosomal aberration assay is emphasized in the evaluation or re-evaluation of the genotoxic invitro effect of xenobiotics, to evidence chromosomal damage.