Reference values for acetyl and butyrylcholinesterases in cattle under actual management conditions, hepatic and renal function by application of chlorpyrifos

DANIELA M. FERRÉ; VALERIA R. LENTINI; R. RAQUEL ROMANO,; HECTOR R. LUDUEÑA,; PAOLA J. JOTALLÁN,; NORA B. M. GORLA

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH, PART B

Taylor and Francis Online

Año: 2018 vol. 53 p. 191 - 198

1532-4109

Chlorpyrifos is an anticholinesterase organophosphate insecticide widely used in Argentina in theproduction of food derived from animal, fruit and horticultural origin and is reported as a residue withinthese products. Local reference values for acetyl and butyrylcholinesterase were determined in AberdeenAngus bovine and cross bred cattle (n D 25), a requirement to be able to evaluate toxicity of commercialorganophosphate and carbamate formulations. The activity of cholinesterase enzymes presented anoverall mean of 2,183.00 § 485.6 IU L¡1 for erythrocyte acetylcholinesterase and 203.1 § 42.06 IU L¡1 forplasma butyrylcholinesterase, which are used as reference values for meat steers within a system ofintensive production in a semi-arid region. The toxic potential of chlorpyrifos in steers of the same breeds(n D 12) was assessed applying chlorpyrifos 15.00% Tipertox in a single therapeutic dose of 7.50 mg kg¡1by topical route. Prior to application and then on day 1 and day 21 post-application, both bloodcholinesterases, serum chlorpyrifos concentration by ultra-high resolution liquid chromatography withmass detector, analysis of blood counts, total proteins, liver enzymes, urea and creatinine were evaluated.The mean plasma concentration of chlorpyrifos was 27.90 ug L¡1 at 24 h. The findings indicate that thetherapeutic treatment of castrated male bovines treated with chlorpyrifos, applied by pour-on accordingto the manufacturer?s instructions, does not cause changes in the variables evaluated.