FERRE daniela Marisol
Reference values for acetyl and butyrylcholinesterases in cattle under actual management conditions, hepatic and renal function by application of chlorpyrifos
DANIELA M FERRÉ; VALERIA LENTINI; ROMANO RAQUEL; R LUDUEÑA; PAOLA JOTALLAN; NORA B GORLA
Journal Journal of Environmental Science and Health, Part B- Pesticides, Food Contaminants, and Agricultural Wastes
Taylor & Francis
Chlorpyrifos is an anticholinesterase organophosphate insecticide widely used in Argentina in the production of food derived from animal, fruit and horticultural origin and is reported as a residue within these products. Local reference values for acetyl and butyrylcholinesterase were determined in Aberdeen Angus bovine and cross bred cattle (n = 25), a requirement to be able to evaluate toxicity of commercial organophosphate and carbamate formulations. The activity of cholinesterase enzymes presented an overall mean of 2183.00 ± 485.6 IU / L for erythrocyte acetylcholinesterase and 203.1 ± 42.06 IU / L for plasma butyrylcholinesterase, which are used as reference values for meat steers within a system of intensive production in a semi-arid region. The toxic potential of chlorpyrifos in steers of the same breeds (n = 12) was assessed applying chlorpyrifos 15.00% Tipertox® in a single therapeutic dose of 7.50 mg / kg by topical route. Prior to application and then on day 1 and day 21 post-application, both blood cholinesterases, serum chlorpyrifos concentration by UHPLC ms-ms, analysis of blood counts, total proteins, liver enzymes, urea and creatinine were evaluated. The mean plasma concentration of chlorpyrifos was 27.90 ug / L at 24 h. The findings indicate that the therapeutic treatment of castrated male bovines treated with chlorpyrifos, applied by pour-on according to the manufacturer´s instructions, does not cause changes in the variables evaluated.