EVALUATION OF IMIQUIMOD LOADED ARCHAEOSOMES AS POTENTIAL IMMUNOTHERAPY FOR CHAGAS DISEASE

PARRA FL; ALLIANI B; FRANK FM; ROMERO EL; PETRAY PB

CABA

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Eder L Romero (1), Patricia B Petray (2)

Archaeosomes prepared with ether lipids of Archaeobacteria constitute a novel family of liposome. They show high adjuvant activity and can be used as delivery system. We evaluate the immunotherapeutic effect of imiquimod loaded archaeosomes (IMQ-ARC) against experimental T. cruzi infection. C3H/HeN mice were injected ip with trypomastigotes RA and were administered with free IMQ (1mg/kg), IMQ-ARC, empty ARC or PBS. Treatment was carried out sc on the back at day 0 and 7 pi. Other group received BZ (100mg/kg/day) by gavage as reference therapy for 14 consecutive days. Parasitemia, body weight and mortality were registered. Survival mice were euthanized at 60 dpi and serum samples were obtained to assess the serological profile. Fragments of heart and skeletal muscle were collected for histopathological analysis. The results showed that treatment with IMQ-ARC provoked a significant decrease (p