INVESTIGADORES
FRANK fernanda Maria
congresos y reuniones científicas
Título:
A Brucella spp. protease inhibitor is a useful adjuvant in oral vaccine formulations against infectious diseases.
Autor/es:
CARABAJAL MV; IBAÑEZ AE; RISSO GS; CORIA LM; GONZALEZ COBIELLO PL; BRUNO L; FRANK FM; BRIONES G; PASQUEVICH KA; CASSATARO J
Lugar:
Berlin
Reunión:
Congreso; Brucellosis 2014 International Research Conference Including the 67th Annual Brucellosis Research Meeting; 2014
Institución organizadora:
Federal Institute for Risk Assessment (Bundesinstitut für Risikobewertung, BfR) in cooperation with the Federal Research Institute for Animal Health (Friedrich-Loeffler-Institut, FLI)
Resumen:
Introduction: Previous results showed that a Brucella spp. protein (BP) administered by the oral route and without adjuvants induced protection against oral Brucella challenge. BP is a broad spectrum protease inhibitor with a stronger activity against serine proteases secreted by the pancreas to the intestine (elastase, trypsine and alpha chymotrypsin). These previous results prompted us to study its potential application as oral adjuvant for other antigens (Ags). Methods and Results: As BP is able to inhibit in vitro the activity of many proteases present at the gastrointestinal tract we directed all our efforts to study its potential application in oral vaccine delivery. We showed that in BALB/c or C57BL/6 mice oral delivery of OVA in the presence of BP resulted in a significantly increased OVA-specific T cell response (DTH assay; P