EVALUATION OF ANGIOGENIC EFFECTS OF LITHIUM-MODIFIED 45S5 BIOACTIVE GLASS

VARGAS GE, HARO DURAND LA, VERA MESONES R, FANOVICH, MA, GORUSTOVICH A

Chascomús

Jornada; XV Jornadas Anuales de la Sociedad Argentina de Biología (2013); 2013

Sociedad Argentina de Biología

The controlled and localized release of lithium (Li) ions from bioactive glasses (BGs) might prove a promising therapeutic alternative to initiate angiogenesis in tissue repair procedures. The aim of this work was to assess the angiogenic effects of the ionic dissolution products (IDPs) of new BGs based on 45S5-type glass doped with 1% (45S5.1Li) or 5% Li2O (45S5.5Li). The IDPs were obtained by incubating 1% w/v particles (<5 µm) of BGs in egg water at 37ºC for 72 h. The determination of soluble ions was conducted through ICP-MS. Angiogenesis assays were carried out on zebrafish embryos (Danio rerio) at 48 hpf. The embryos were incubated for 24 h at 28.5°C in 6-well culture plates containing either 5 mL egg water (control) or egg water enriched with the IDPs. We carried out 2 replicates with n=30 embryos per treatment. The embryos were processed for the subsequent morphometric analysis of the subintestinal vascular plexus, through enzyme-histochemical identification of endogenous alkaline phosphatase. In all cases we observed the embryonic development expected for the incubation time assessed. The results obtained showed that only the IDPs of 45S5.5Li BG exhibited pro-angiogenic capability. The positive effects can be attributed to the release of 0.20 mM Li from 45S5.5Li microparticles.