Angiogenic potential of ionic dissolution products from a new bioactive glass?ceramic material

HARO DURAND L, GIMENO M, GOMEZ GRAMAJO F, VARGAS G, VERA MESONES R, FANOVICH A, BOCCACCINI A, PERONE M, GORUSTOVICH A

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Congreso; 3er Congreso Argentino de Osteología. Asociación Argentina de Osteología y Metabolismo Mineral (AAOMM) y Sociedad Argentina de Osteoporosis (SAO); 2020

Argentinian Society of Osteology and Mineral Metabolism (AAOMM)

This study aimed to evaluate the in vivo neovascularization of Geltrex implants enriched with ionic dissolution products (IDPs) from scaffolds obtained from 45S5 bioglass containing 2% B2O3 (45S5.2B) under experimental diabetes mellitus (DM). IDPs were obtained by incubating scaffolds for 72 h in DPBS.Type-I DM was established using the adoptive transfer model in NOD-scid mice, assigned to the following groups: Control Group (n=5), non-compensated DM Group (n=5), and insulin-compensated DM Group (n=5). Under anesthesia, the mice were dorso-laterally s.c. injected with 500 µL of Geltrex containing DPBS enriched with IDPs in combination with bFGF. DPBS + bFGF was used as positive control. In each animal, the positive control was injected on one side and the problem sample on the other side. At 7 d post-treatment, the mice were euthanized and the Geltrex masses removed and then processed for further histological evaluation and flow cytometry. The microscopic study demonstrated the neovascularization of the implants enriched with the IDPs from the 45S5.2B scaffolds in the Control and compensated DM mice. Flow cytometry results showed an 85% increase in the number of CD31+CD45- endothelial cells in Geltrex samples enriched with the IDPs from the 45S5.2B scaffolds implanted in the insulin-compensated DM Group compared to the response observed in the non-compensated DM mice, which showed a marked decrease (72%) in the number of endothelial cells with respect to the control animals. The results obtained may have therapeutic relevance in DM patients with critical limb ischemia, as the IDPs from 45S5.2B scaffolds could act as inorganic agents with the ability to enhance tissue neovascularization.