Protein kinase activities in Bufo arenarum embryos exposed to azinphosmethyl and carbaryl

ANA FERRARI; ANDRÉS VENTURINO; ANA M PECHÉN DE D'ANGELO

Rosario, Santa Fé, Argentina

Congreso; XLII Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas y Biología Molecular (SAIB); 2006

SAIB

Most relevant efforts to understand pesticide actions are presently directed to cellular signaling and gene response. We evaluate here the effects of azinphosmethyl (AM, 9mg/L) and carbaryl (CB, 20mg/L) on basal protein kinase, PKC and PKA activities in Bufo arenarum larvae after continuous exposure from fertilization. The activity of the oxidative stress- responding enzyme GST was also evaluated. Kinase assay (32PgATP; Histone) conditions were fitted using adult toad brain. Control larvae showed a significant basal kinase (30.2 dpm/µg protein) and PKA (cAMP-activated phosphorylation; 12.7 dpm/µg protein) activity, but PKC (DAG/PC-activated phosphorylation) was absent at this stage. Exposure to AM caused a significant increase of basal kinase (199%) and PKA (192%), and activated PKC (22.6 dpm/µg protein). Exposure to CB only produced a significant increase in basal kinase activity (124%). Larvae showed an induction of GST activity due to AM exposure (71%) and to a lesser extent to CB (33%) (p