In vivo and in vitro atrazine evaluation on digestive enzymes of eared dove (Zenaida auriculata)

MANZANOS, N.; BACH, N.; CID, F. D.; CHEDIACK, J. G.

Mendoza

Congreso; XL Reunión Anual de a Sociedad de Biología de Cuyo; 2022

Sociedad de Biología de Cuyo

It has been observed that certain environmental pollutants can affect the enzymatic activity of the digestive system in some vertebrates. However, this effect is poorly studied in birds. Atrazine is a widely used herbicide in our country, and negative effects on the physiology of some organisms have been described. In these sense, although atrazine has been shown to produce some adverse effects in birds, there is a lack of knowledge about the effect on digestive physiology. Thus, the main objective of this work was to elucidate the Atrazine effects on digestive enzymes (intestinal and pancreatic) in a in vivo model of Eared Dove and the direct effect in brush border membrane vesicles (BBMV), in vitro model. To achieve our goal related to in vivo experiment, we established three independent groups of birds (n=6 each group), two groups were exposed during 15 days to the 25 mg/kg and 250 mg/kg atrazine concentration. After exposure, body weight measure, blood extraction and removal of the intestine, stomach, liver and pancreas were performed at the same time (8:00 am), to avoid disturb by circadian/daily variation. BBMV was obtained after intestinal lumen scraping using a polyethylene glycol (PEG) technique, then, BBVM were exposed to Atrazine concentrations of 5 µM; 2.5 µM; 1 µM and 0.5 µM, taking into account two controls, one withbuffer and the other with ethanol, since atrazine is solubilized at 0.4% in ethanol. The intestinal enzymatic activity of sucrase, maltase and aminopeptidase was determined in both in vivo and in vitro models and the enzymatic activity of the pancreatic enzymes trypsin and chymotrypsin was determined in vivo model. Statistical analysis performed was RM-ANOVA to compare the proximal, medial, and distal portions of the in-vivo model and for pancreatic enzymes. In-vitro model was analyzed by one-way ANOVA, Tukey's post-hoc test (p