Production of mixed biofilm between Candida albicans and streptococcus equi zooepidemicus isolated from vagina of horses

GUIDA NORA; ETCHECOPAZ ALEJANDRO; BUSTOS CARLA; MUÑOZ ALEJANDRA

Valencia

Jornada; 5th Trends in Medical Mycology; 2011

EORTC

Production of mixed biofilm between Candida albicans and Streptococcus equi zooepidemiccus isolated from vagina of horses Nora Guida, Alejandro N. Etchecopaz, Carla Bustos, Alejandra J  Muñoz In all mucous, a resident microbiotic exists as consequence of ecological successions that selected the species more adapted to the site. The bacteria and fungi’s community is dynamic and diverse, and it can be modified with the environmental disturbance generating beneficial or harmful interactions to mares. It is suggested that the molecular mechanisms rule the physical associations, the communication inter spices, changes of the microbial behavior and the survival that happens when the bacteria and fungi occupy the same sites. The cooperative effects observed in infections caused for eukaryotes and prokaryotes might be due to the formation mixed biofilms. This growth it can promote the resistance to antimicrobial drugs. Uterine infections in not pregnant mares have become frequently by the widespread use of antibiotics and the manipulation in the intensive reproduction. Streptococcus equi zooepidemiccus, (Sez ) is the most frequently isolated bacterium of endometritis. It is considered a part-mucosal of the vagina commensally. On the other hand the Candida (C) is isolated of vagina and uterus too, but  the relations with the clinical uterine disease are confused because the  recovery in culture is  more frequent than it participations in the etiology of vaginal or uterine disease The aim of this study was to standardize a technology to evaluate the biofilm formation of to reference laboratory strain of Sez and  C. Albicans,  and strains isolated of horses  separately and compounds biofilms. The first objective was to verify if the bacteria and candida can produce biofilms on abiotic (glass) surfaces, and we developing and optimizing a reproducible associated biofilm model. Petri´s plates of glass was used with sterile cover glass and added with fetal equine serum, incubating for 1 hour at 37 ° C, washed with Broth Tripteina Soja (Britannia®) (CTS), containing 5 % of equine serum . They were in use 150 ul of Ca´s suspension (5×106 blastspores/ml) and 150 ul of Sez suspension.5×106 bacteria/ml). The plates were incubated for 1 hour at 37 ° C. The cover glasses were washed two times with CTS to remove the planktonic’s cells and they were incubated for 24 hs at 37 ° C. Then they were washed three times with PBS. In order to better visualize biofilm samples, we used the cationic dye alcian blue by addition of 0.1% alcian blue. Close examination of biofilm structures showed congregating Sez, Ca and biofilm mixture onto the glass surface. These findings suggest that glass could act as substrates for adhesion and, possibly, growth as biofilms, structured microbial communities, C albicans and Sez embedded in an extracellular polymeric substance. The study of the behavior between bacteria and fungi that coexist in horse’s mucous, in biofilms, will help to treat the polymicrobial opportunists infections with efficiency.