CONICET | Buscador de Institutos y Recursos Humanos

Bioaerosols: Study of the bacteria and the fungical quality of the exhaled air by equine. We define like bioaerosols to any particle transported by the air, constituted by big molecules that have been liberated to air. Most of the bacteria behave as optional parasites being able to live and to develop in organisms or on organic matter. The commission for the bioaerosols of the ACGIH (American Conference of Governmental Industrial Hygienists) explains the reasons for which though it is not possible to establish uniform criteria to value for quantitative form the composition of the cultivable bioaerosols, the bacteria and present fungi in the air exhaled by animals and human beings it allows us to know the composition of bioaerosols cultivable exhaled. Objective: To study the bacterial aerobic composition and cultivable fungi of the air exhaled for different equine. Materials and methods: 8 animals were studied to field in corrals of land and fed on bale. The samples were obtained placing plates of Petri opened with agar Tripteine soja with 10% of equine blood and agar Saboureaud 2% glucosado with 0,05% of enrofloxacina and other one with 0,05 of enrofloxacina and 0,05% of cicloheximida, to 5cm, approximately, of distance of the Orifice nasals of the animal during 4 respiratory movements. The measured concentrations of the cultivable bioaerosols depend on the method of capture of sample and analysis. It is not possible to gather and to evaluate all the components of the bioaerosols using the only method of sampling. We choose for this test the sampling of exhaled air. The cultures were incubated to 35-37°C and 25-28°C during 24-48 h for bacteria and up to 30 days for fungi. There were studied the macroscopic characteristics of the bacterial colonies and were observed microscopically by means of Gram´s coloration. There were selected colonies different from every plate obtaining 16 isolations. The fungi were studied for the macrocolonis and microcultivos method. Results: In all the plates of culture used for the sampling bacterial colonies were Gstraisrowth. Using bichemical test they could be identified: 8 strains of negative coagulasa Staphylococcus, 6 strains of Corynebacterium spp and 2 Bacillus spp. Fungi was isolated in 100% of the animals. The composition of the mycological population found in the exhaled air was changed, different kind being identified as Aspergillus spp, Penicillum spp, Cladosporium spp, Alternaria spp, ann Mucor spp, between others.

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