INVESTIGADORES
CENTRON Daniela
artículos
Título:
Molecular fingerprinting of Staphylococcus aureus isolated from patients with osteomyelitis in Argentina and clonal distribution of the cap5(8) genes and of other selected virulence genes
Autor/es:
LATTAR SM, TUCHSCHERR LP, CENTRÓN D, BECKER K, PREDARI SC, BUZZOLA FR, ROBINSON DA, SORDELLI DO.
Revista:
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES : OFFICIAL PUBLICATION OF THE EUROPEAN SOCIETY OF CLINICAL MICROBIOLOGY.
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2012 vol. 31 p. 2559 - 2566
ISSN:
0934-9723
Resumen:
The molecular fingerprinting of a collection of 94
Staphylococcus aureus isolates from patients with osteomyelitis
in Argentina was performed. Twenty-three SmaI
pulsed-field gel electrophoresis (PFGE) types and 37 spa
types were identified. The isolates were assigned to 23
sequence types (STs). The proportion of methicillinresistant
S. aureus (MRSA) isolates was significantly higher
among cap5 S. aureus (35/61) compared with cap8 S.
aureus (8/33) isolates (p00.0025). Twenty-four of the 94
isolates carried the lukS-PV/lukF-PV genes, which were
significantly associated to cap5 [(23/38) compared with
cap8 S. aureus isolates (1/32) (p00.0001)]. Forty of the 94
isolates carried genes of the egc locus (seg/sei). The distribution
of seg/sei genes among isolates was related to certain
clones. Isolates of the four agr types were found in the S.
aureus collection. Whereas agr I isolates were evenly distributed
among cap5 and cap8 S. aureus isolates (32/61 and
14/33, respectively), the agr II group was composed of 29
cap5 S. aureus isolates and agr III was composed of 16 cap8
S. aureus isolates. Two clones originally associated to animals
(ST 188, 7 isolates and ST 1796, 5 isolates) were
associated with chronic osteomyelitis and lack of capsular
polysaccharide (CP) production. Loss of CP production
remains the single factor among those investigated that is
associated with chronic osteomyelitis.