INVESTIGADORES
CENTRON Daniela
artículos
Título:
Diversity and strength of internal outward-oriented promoters in group IIC-attC introns.
Autor/es:
LÉON G, QUIROGA C, CENTRÓN D, ROY PH.
Revista:
NUCLEIC ACIDS RESEARCH
Editorial:
OXFORD UNIV PRESS
Referencias:
Lugar: Oxford; Año: 2010
ISSN:
0305-1048
Resumen:
Integrons are genetic elements that incorporate
mobile gene cassettes by site-specific recombination
and express them as an operon from a
promoter (Pc) located upstream of the cassette insertion
site. Most gene cassettes found in integrons
contain only one gene followed by an attC recombination
site. We have recently shown that a specific
lineage of group IIC introns, named group IIC-attCattC recombination
site. We have recently shown that a specific
lineage of group IIC introns, named group IIC-attCattC
introns, inserts into the bottom strand sequence of
attC sites. Here, we show that S.ma.I2, a group IICattCsites. Here, we show that S.ma.I2, a group IICattC
intron inserted in an integron cassette array of
Serratia marcescens, impedes transcription from Pc
while allowing expression of the following antibiotic
resistance cassette using an internal outwardoriented
promoter (Pout). Bioinformatic analyses
indicate that one or two putative Pout, which have
sequence similarities with the Escherichia coli consensus
promoters, are conserved in most group
IIC-attC intron sequences. We show that Pout with
different versions of the 35 and 10 sequences are
functionally active in expressing a promoterless
chloramphenicol acetyltransferase (cat) reporter
gene in E. coli. Pout in group IIC-attC introns may
therefore play a role in the expression of one or
more gene cassettes whose transcription from Pc
would otherwise be impeded by insertion of the
intron., impedes transcription from Pc
while allowing expression of the following antibiotic
resistance cassette using an internal outwardoriented
promoter (Pout). Bioinformatic analyses
indicate that one or two putative Pout, which have
sequence similarities with the Escherichia coli consensus
promoters, are conserved in most group
IIC-attC intron sequences. We show that Pout with
different versions of the 35 and 10 sequences are
functionally active in expressing a promoterless
chloramphenicol acetyltransferase (cat) reporter
gene in E. coli. Pout in group IIC-attC introns may
therefore play a role in the expression of one or
more gene cassettes whose transcription from Pc
would otherwise be impeded by insertion of the
intron.out). Bioinformatic analyses
indicate that one or two putative Pout, which have
sequence similarities with the Escherichia coli consensus
promoters, are conserved in most group
IIC-attC intron sequences. We show that Pout with
different versions of the 35 and 10 sequences are
functionally active in expressing a promoterless
chloramphenicol acetyltransferase (cat) reporter
gene in E. coli. Pout in group IIC-attC introns may
therefore play a role in the expression of one or
more gene cassettes whose transcription from Pc
would otherwise be impeded by insertion of the
intron.out, which have
sequence similarities with the Escherichia coli consensus
promoters, are conserved in most group
IIC-attC intron sequences. We show that Pout with
different versions of the 35 and 10 sequences are
functionally active in expressing a promoterless
chloramphenicol acetyltransferase (cat) reporter
gene in E. coli. Pout in group IIC-attC introns may
therefore play a role in the expression of one or
more gene cassettes whose transcription from Pc
would otherwise be impeded by insertion of the
intron.Escherichia coli consensus
promoters, are conserved in most group
IIC-attC intron sequences. We show that Pout with
different versions of the 35 and 10 sequences are
functionally active in expressing a promoterless
chloramphenicol acetyltransferase (cat) reporter
gene in E. coli. Pout in group IIC-attC introns may
therefore play a role in the expression of one or
more gene cassettes whose transcription from Pc
would otherwise be impeded by insertion of the
intron.attC intron sequences. We show that Pout with
different versions of the 35 and 10 sequences are
functionally active in expressing a promoterless
chloramphenicol acetyltransferase (cat) reporter
gene in E. coli. Pout in group IIC-attC introns may
therefore play a role in the expression of one or
more gene cassettes whose transcription from Pc
would otherwise be impeded by insertion of the
intron.cat) reporter
gene in E. coli. Pout in group IIC-attC introns may
therefore play a role in the expression of one or
more gene cassettes whose transcription from Pc
would otherwise be impeded by insertion of the
intron.E. coli. Pout in group IIC-attC introns may
therefore play a role in the expression of one or
more gene cassettes whose transcription from Pc
would otherwise be impeded by insertion of the
intron.