Genomic analysis of two Acinetobacter baumannii strains belonging to two different sequence types (ST172 and ST25)

MONTAÑA S; VILACOBA E; FERNANDEZ JS; TRAGLIA GM; SUCARI A; PENNINI M; IRIARTE A; CENTRON D; MELANO RG; RAMÍREZ MS

J Glob Antimicrob Resist

Elsevier

Año: 2020

2213-7165

Objective: Acinetobacter baumannii is an opportunistic nosocomial pathogen that is the main focus of attention in clinical settings, due to its intrinsic ability to persist in hospital environment and its capacity to acquire determinants of Journal Pre-proofresistance and virulence. Here, we present the genomic sequencing, the molecular characterization, and genomic comparison of two A. baumannii strains, that belong to two different STs, one sporadic and one widely distributed in our region. Methods: The whole genome sequence (WGS) of Ab42 and Ab376 were obtained using Illumina MiSeq- I and assembled with SPAdes. ARG-ANNOT, CARD-RGI, ISfinder, PHAST, Plasmid Finder, PlasmidSpades and Islandviewer were used to analyze both genomes. Results: Genome analysis revealed that Ab42 belongs to ST172, an uncommonST, while Ab376 belongs to ST25 a widely distributed ST. The molecular characterization showed the presence of two antibiotic resistance genes in Ab42 and nine in Ab376. No insertion sequences were detected in the Ab42, however, 22 were detected in Ab376. Moreover, two prophages were found in Ab42 and three in Ab376. In addition, a CRISPR-cas type I-Fb and two plasmids, one of which harbored an AbGRI1-like island, were found in Ab376. Conclusion: We present the WGS analysis of two A. baumannii strains that belong to two different STs. These findings allowed us to characterize a not previously described ST (ST172) and provide new insights to the widely studied ST25.