INVESTIGADORES
GALDEANO ernestina
artículos
Título:
Establishment of the new subgroup 16SrI-S (rr-rp) tuf-H belonging to ‘Ca. Phytoplasma asteris’ in wild and cultivated plants in Argentina
Autor/es:
L.E. TORRES; MENEGUZZI, N.G; FERNÁNDEZ, F; GALDEANO, E; CONCI, L
Revista:
JOURNAL OF PLANT PATHOLOGY
Editorial:
EDIZIONI ETS
Referencias:
Año: 2011 vol. 93 p. 311 - 320
ISSN:
1125-4653
Resumen:
Argentinian catharanthus little leaf phytoplasma (ACLL) was found naturally infecting Daucus carota L. (carrot), Catharanthus roseus (LG Don) (periwinkle) and wild Matricaria chamomilla L. (chamomile). All infected plants showed symptoms of yellowing, little leaf and internodes shortening. In particular, flower escape distortion and malformation of umbels and roots were observed in infected carrots, witches´-broom in chamomile, while only periwinkle plants showed virescence and phyllody. On the basis of restriction fragment length polymorphism (RFLP) analyses of partial 16S rDNA, ribosomal protein rpl22 and tuf (EF-Tu) genes, ACLL phytoplasma was classified in group 16SrI (‘Ca. Phytoplasma asteris’), new subgroup 16SrI-S (rr-rp) tuf-H. All the strains of ACLL phytoplasma have 16S rRNA interoperon sequence heterogeneity as shown by PCR-RFLP and nucleotide sequence analyses. Phylogenetic analysis using 16S rDNA sequences clustered ACLL phytoplasma strains into a new phylogenetic lineage within group 16SrI.