INVESTIGADORES
MARTINEZ eric Javier
artículos
Título:
Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
Autor/es:
MARTÍNEZ, ERIC J.; HOPP, HORACIO E.; STEIN, JULIANA; ORTIZ, JUAN P. A.; QUARIN, CAMILO L.
Revista:
MOLECULAR BREEDING
Editorial:
Springer Netherlands
Referencias:
Año: 2003 vol. 12 p. 319 - 327
ISSN:
1380-3743
Resumen:
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Tetraploid Paspalum notatum bahiagrass
is a valuable forage grass with aposporous apomictic reproduction. In a previous
study, we showed that apospory in bahiagrass is under the control of a single
dominant gene with a distorted segregation ratio. The objective of this work
was to identify molecular markers linked to apospory in tetraploid P.
notatum and establish a preliminary syntenic relationship with the genomic
region associated with apospory in P. simplex. A F1 population of 290
individuals, segregating for apospory, was generated after crossing a
completely sexual plant Q4188 with a natural aposporous apomictic plant Q4117. The
whole progeny was classified as sexual or aposporous by embryo sacs analysis. A
bulked segregant analysis was carried out to identify molecular markers
co-segregating with apospory. Four hundred RAPD primers, 30 AFLP primers
combinations and 85 RFLP clones were screened using DNA from both parental
genotypes and aposporous and sexual bulks. Linkage analysis was performed with
cytological and genetic information from the complete progeny. Cytoembryological
analysis showed 219 sexual and 71 aposporous F1 individuals. Seven different
molecular markers 2 RAPD, 4 AFLP and 1 RFLP were found to be completely linked
to apospory. The RFLP probe C1069, mapping to the telomeric region of the long
arm of rice chromosome 12, was one of the molecular markers completely linked
to apospory in P. notatum. This marker had been previously associated
with apospory in P. simplex. A preliminary map of the chromosome region
carrying the apospory locus was constructed.
Paspalum
notatum bahiagrass is a valuable forage grass with
aposporous apomictic reproduction. In a previous study, we showed that apospory
in bahiagrass is under the control of a single dominant gene with a distorted
segregation ratio. The objective of this work was to identify molecular markers
linked to apospory in tetraploid P. notatum and establish a preliminary
syntenic relationship with the genomic region associated with apospory in P.
simplex. A F1 population of 290 individuals, segregating for apospory, was
generated after crossing a completely sexual plant Q4188 with a natural
aposporous apomictic plant Q4117. The whole progeny was classified as sexual or
aposporous by embryo sacs analysis. A bulked segregant analysis was carried out
to identify molecular markers co-segregating with apospory. Four hundred RAPD
primers, 30 AFLP primers combinations and 85 RFLP clones were screened using
DNA from both parental genotypes and aposporous and sexual bulks. Linkage
analysis was performed with cytological and genetic information from the
complete progeny. Cytoembryological analysis showed 219 sexual and 71
aposporous F1 individuals. Seven different molecular markers 2 RAPD, 4 AFLP and
1 RFLP were found to be completely linked to apospory. The RFLP probe C1069,
mapping to the telomeric region of the long arm of rice chromosome 12, was one
of the molecular markers completely linked to apospory in P. notatum.
This marker had been previously associated with apospory in P. simplex.
A preliminary map of the chromosome region carrying the apospory locus was
constructed.