INVESTIGADORES
PORTIANSKY enrique Leo
artículos
Título:
Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate.
Autor/es:
FERNANDEZ PE; SANGUINETI HR; PORTIANSKY EL; BARBEITO CG; GIMENO EJ
Revista:
PESQUISA VETERINáRIA BRASILEIRA
Editorial:
REVISTA PESQUISA VETERINARIA BRASILEIRA
Referencias:
Año: 1999 vol. 19 p. 133 - 138
ISSN:
0100-736X
Resumen:
The immunodetection of diverse cell markers was evaluated in prostatic samples frombullocks, and bullocks showing epithelial hyperplasia-metaplasia, with oestrogen-induced changes, and in experimental samples from bullocks inoculated with dietylstilbestrol (DES). Antigen-retrieval procedures allowed the use of tissues that had been fixed in formalin for long periods. Three tissue markers were chosen for the study: cytokeratins 13 and 16, vimentin and desmin. Monoclonal antibody K8.12 (specific for cytokeratins 13 and 16) stained basal cells and hyperplastic-metaplastic epithelium; monoclonal antivimentin, and desmin, allowed the definition of fibromuscular changes
