Increase of murine splenic natural antibodies secreting cells after cyclophosphamide treatment

PORTIANSKY EL; GONZALEZ PH; LAGUENS RP

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY

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Año: 1996 vol. 81 p. 215 - 217

0090-1229

Administration of a low sub-immunosuppressive widely unrelated Ag. dose of cyclophosphamide (CY) to naive mice induced a marked increase in the number of splenic cells form- MATERIALS AND METHODS ing natural antibodies against unrelated antigens such as foot-and-mouth disease virus, keyhole limpet hemo- Animals. Adult outbreeding 60-day-old male Swiss cyanin, horseradish peroxidase, or bovine serum albu- mice were used for the collection of B lymphocytes from min, as determined by an enzyme-linked immunosor- the spleen. bent assay spot technique. These results suggest thatin mice there exists a repertoire of B cells forming Virus. FMDV strain O1 Caseros was purified acnatural antibodies which is restrained by an unknown cording to Brown and Cartwright (3). mechanism and that CY is able to interfere with that restriction.